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PUC19

pUC19 gehört wie auch pUC18 zu einer Reihe im Labor von Joachim Messing gentechnologisch hergestellter Klonierungsvektoren. Diese bakteriellen Plasmide gehören zu den pUC19 Vector. pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and

pUC19 - Wikipedi

  1. pUC19 Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC18
  2. pUC19 und pUC18 sind künstlich hergestellte, bakterielle Plasmide, die zu den am häufigsten verwendeten Vektoren zur Klonierung und Expression von Proteinen im
  3. Plasmid-DNA pUC19 lyophilisiert. OD 260/280: >1,70 Genom. DNA: <2 % oc-Form: <3 % ccc-Form: >95 % Basenpaare: 2686. pUC ist ein gängiger high-copy Klonierungsvektor
  4. Full Sequences from Depositor (1) Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results. Discrepancies
  5. puc19 sequence (2686 bp) 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121
  6. pUC19 Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning

pUC19 Vector NE

pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. The designation pUC is derived from the classical p prefix (denoting pUC19 pUC19 is a small, high-copy number E. coli plasmid cloning vector containing portions of pBR322 and M13mp19 (1). It con-tains the pMB1 origin of replication

pUC19. In red is the bla gene, with lacZα in yellow and the origin of replication in green <protect> Type Cloning Origin of Replication pMB1 (mutant) ColE1 Copy # Die Vektoren pUC18/ pUC19 aus E.coli sind standardmäßig benutzte, kleine high copy number E. coli Plasmide von 2686 bp Länge. pUC19 ist identisch mit pUC18, außer The pUC18 and pUC19 plasmids enable successful cloning of large DNA fragments (larger than those cloned with a M13 mp18 RF Phage Vector). These cloning vectors contain Title: pUC19 Linearized Vector Map Author: Clontech Laboratories, Inc. Subject: The pUC19 Linearized Vector is provided as a part of In-Fusion® Cloning Kits, and is Using clean, supercoiled pUC19, the efficiency is highest in the 100 pg-1 ng range. However, the total colonies which can be obtained from a single reaction increase

Thermo Scientific pUC19 DNA/MspI (HpaII) Marker is recommended for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and pUC19 is a small, high-copy number E. coli plasmid cloning vector, of which multiple cloning sites as shown below. The molecule is a small double-stranded circle, 2686

The Methylated & Non-methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers. The set is ideal as a spike-in control to assess pUC19 gehört wie auch pUC18 zu einer Reihe im Labor von Joachim Messing gentechnologisch hergestellter Klonierungsvektoren.[1] Diese bakteriellen Plasmide gehören zu den am häufigsten verwendeten Vektoren zur Klonierung im Bakterium Escherichia coli. Damit haben sie in der biologischen Forschung und Gentechnik eine große Bedeutung. Ihr spezieller Vorteil sind die kleine Größe, die. pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number. pUC19 carries a 54 base-pair multiple cloning site polylinker that contains unique sites for 13 different hexanucleotide-specific restriction endonucleases (1)

The higher copy number of pUC19, compared to its parent plasmid pBR322, is known to be due to deletion of rop, also known as rom, and to an ori mutation that impedes RNAI:RNAII interaction. pUC19, unlike pBR322, fails to transform E. coli rho mutant rho026 cells. Here we identify two features of pUC Plasmid pUC19 (0.8 ng/μL) were added to the cell suspension and then treated with ultrasound, thereafter SOC medium was added and the mixture was incubated at 37 °C for 1 h. 100 μLof mixture was spread onto LB-Amp (100 μg/mL) agar plate and incubated overnight at 37 °C to score for transformants. Each point was the average of 5 plates and calculated the transformation efficiency, which. pUC19 vector backbone. + Sequence information. + Datasheet. + Compare & Order pUC19 vector backbone products + TOP customer support pUC19 is a small, high copy cloning vector for replication in E. coli.It has been constructed using the ampicillin resistance gene and the pMB1 origin of replication from pBR322.The pMB1 of pUC19 differs from the pBR322 origin by a single point mutation and the lack of the rop gene, leading to a high copy number. pUC19 has a multiple cloning site within the lacZ alpha-fragment

3.7.2.2 Plasmid-Vektor pUC19 pUC19 setzt sich aus Teilen des Plasmids pBR322 und des Bakteriophagen M13mp19 zusammen. Das 2,683 kb große Plasmid besitzt neben dem Replikationsursprung und dem Gen für Ampicillinresistenz auch Teile des lac-Operons von E. coli, worauf die Gene zur Verwertung von Laktose enthalten sind. pUC19 enthält davon die Sequenzen des Repressors, Promotors und Operators. puc19 sequence (2686 bp) 1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc 241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg 301 ttttacaacg. pUC19 Control DNA (10 pg/μL) 50 μL S.O.C. Medium 6 mL PRODUCT INFORMATION SHEET For Research Use Only. Not for use in diagnostic procedures. Important guidelines Handle competent cells gently as they are highly sensitive to changes in temperature or mechanical lysis caused by pipetting. Thaw One Shot™ competent cells on ice, and transform cells immediately following thawing. After adding. 1 Definition. Als Klonierung bezeichnet man die systematische Amplifikation spezifischer DNA-Fragmente.Hierbei ist die gewünschte Vermehrung von Erbsubstanz-Fragmenten in vivo bzw. in vitro möglich.. 2 Klonierungsmechanismus. Für die Umsetzung der Klonierung ist ein Vektor unerlässlich. Dieser erfüllt die Funktion der Übertragung des DNA-Fragmentes in die auserwählte Empfängerzelle

The pUC19 plasmid (2,686 bp) confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains. The multiple cloning site (MCS) is within the B-galactosidase gene; the plasmid has thirteen unique sites in the MCS (Acc I, BamH I, EcoR I, Hinc II, Hind III, Kpn I, Pst I, Sac I, Sal I, Sma I, Sph I, Xba I, and Xma I) NEBcutter V2.0. This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. By default, only enzymes available from NEB are used, but other sets may be chosen The Methylated & Non-methylated pUC19 DNA Set consists of control DNAs and a set of specifically designed primers. The set is ideal as a spike-in control to assess bisulfite conversion efficiency within the same reaction as the sample, or to produce known mixtures of methylated and non-methylated DNA for assay calibration Mobilizable narrow-host-range plasmids were constructed from pUC18 and pUC19 by addition of a segment of pSUP2021 bearing the basis of mobilization (bom) site and origin of transfer (oriT) of RP4. One pair of expression vectors, pARO180 and pARO190, retains the beta-lactamase (bla) gene and twelve o ready-to-use, not pre-dyed⮞Irregular DNA-marker for analysis of small DNA fragments. The marker was manufactured using pUC19-DN..

pUC19 is a standard high-copy cloning vector for E.coli recombinants. The position of the Multi Cloning Site (MCS), which is inserted in frameinto the LacZ-gene, enables a blue-white selection of insert containing plasmid DNA by -complemen-tation. The copy number of plasmids per cell depends on the temperature and equals app. 70- 80 at 37 °C and over 200 at 42 °C. The total sequence can be. pUC19 gehört wie auch pUC18 zu einer Reihe im Labor von Joachim Messing gentechnologisch hergestellter Klonierungsvektoren. Diese bakteriellen Plasmide gehören zu den am häufigsten verwendeten Vektoren zur Klonierung im Bakterium Escherichia coli.Damit haben sie in der biologischen Forschung und Gentechnik eine große Bedeutung. Ihr spezieller Vorteil sind die kleine Größe, die. Modern bacterial cloning carriers (eg Puc19) use the blue-white screening system to distinguish colonies (clones) of transgenic cells from those containing the parental carrier. In these vectors, extraneous DNA is inserted into a sequence that encodes an essential part of beta-galactosidase, an enzyme to which results in the formation of a blue color colony on the culture ground that is used. https://www.technologyinscience.blogspot.com pUC19 is 2686bp Plasmid. pUC19 carries antibiotic resistance gene - ampicillin. pUC19 has N-terminal fragment of.. Verify the sequence of each colony by sequencing from the pUC19 backbone using the pUC19-Fwd or pUC19-Rev primer. Reference the sequencing results against the expected genomic sequence to check for the presence of Cas9-induced NHEJ or HDR modifications. Calculate the percentage of editing efficiency as (no. of modified clones)/ (no. of total clones). It is important to pick a reasonable number.

pUC19 DNA (10 pg/µL) 50 µL −80°C S.O.C. Medium 6 mL 4°C or room temperature Guidelines for tranforming cells • Only use BL21(DE3) competent cells for protein expression. To propagate or maintain an expression plasmid, use competent cells that does not carry the gene for T7 RNA polymerase (i.e., TOP10, DH5α). • For best results, thaw each vial of cells only once. Subsequent freeze. Kurs 5 Grundpraktikum Genetik Klassische Experimente der Gentechnologie: DNA-Klonierung Pt. 2 Thomas Hankeln & Christiane Kraemer AG Molekulargenetik & Genomanalyse (iOME Entwicklung qualitativer und quantitativer Methoden zum Nachweis von genetischen Veränderungen (Mutationen, GVO) Von der Gemeinsamen Naturwissenschaftlichen Fakultä Datei:PUC19.svg. Größe dieser Vorschau: 679 × 600 Pixel. Weitere Auflösungen: 272 × 240 Pixel | 544 × 480 Pixel | 870 × 768 Pixel | 1.160 × 1.024 Pixel | 820 × 724 Pixel. Diese Datei stammt aus Wikimedia Commons und kann von anderen Projekten verwendet werden. Die Beschreibung von deren Dateibeschreibungsseite wird unten angezeigt ID PUC19 preliminary; circular DNA; SYN; 2686 BP. XX AC X02514; M11662; M77789; ATCC37254; XX DT 10-AUG-1990 (Rel. 5, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE E. coli phagemid vector pUC19 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RP 1-2686 RC M13mp18 from M13mp8 RC M13mp19 from M13mp9 RC pUC18 from pUC8.

pUC19 Sequence and Ma

E. coli Genetic Resources at Yale CGSC, The Coli Genetic Stock Cente Dr A. FATM

pUC19 has up to 3.5 fold greater transformation efficiency when plasmids are delivered at 17 kV/cm using electroporation (18). This is consistent with previous studies in which the relationship between plasmid size and plasmid uptake was analyzed (3, 8, 11, 18). In this study, we compare transformation efficiency of pUC19 and pBR322 in E. coli at varying calcium concentrations. The maximum. Schematische Plasmidkarte von pUC19. Die Gene für die Ampicillinresistenz, den Replikationsursprung sowie der Polylinker (blau) sind eingezeichnet. pUC19 gehört wie auch pUC18 zu einer Reihe im Labor von Joachim Messing gentechnologisch hergestellter Klonierungsvektoren. 26 Beziehungen

pUC19 und pUC18 sind künstlich hergestellte, bakterielle Plasmide, die zu den am häufigsten verwendeten Vektoren zur Klonierung und Expression von Proteinen im Bakterium Escherichia coli gehören. Damit haben sie in der biologischen Forschung und Gentechnik eine große Bedeutung. Ihr spezieller Vorteil ist eine besonders hohe Anzahl von Kopien pro Bakterienzelle (sogenannte high copy number. DNA Sequences and Maps Tool. The nucleotide sequence files available below are those used to produce the plasmid vector, viral and bacteriophage maps contained in New England Biolabs Catalog as well as the tables containing the locations of sites. Maps and location of sites are PDF files. Sequence files are in FASTA or/and GenBank format

pUC19 - Biologi

File:PUC19.svg. Size of this PNG preview of this SVG file: 679 × 600 pixels. Other resolutions: 272 × 240 pixels | 544 × 480 pixels | 870 × 768 pixels | 1,160 × 1,024 pixels | 2,320 × 2,048 pixels | 820 × 724 pixels. This is a file from the Wikimedia Commons. Information from its description page there is shown below Locate commercially available restriction enzymes by category, name, recognition sequence, or overhang

Plasmid-DNA pUC19 Plasmid-DNA DNA-/RNA-Analyse

English: scheme of pUC19 plasmid. Deutsch: Schema des Plasmides pUC19. Datum: 19. November 2010: Quelle: Eigenes Werk; NEB: Urheber: Yikrazuul: Lizenz. Public domain Public domain false false: Ich, der Urheberrechtsinhaber dieses Werkes, veröffentliche es als gemeinfrei. Dies gilt weltweit. In manchen Staaten könnte dies rechtlich nicht möglich sein. Sofern dies der Fall ist: Ich gewähre. Ti Plasmid. The Tumour inducing or Ti plasmid is present in the bacterium Agrobacterium tumifaciens. It is widely used now as a cloning vector to deliver desirable genes to the host plant to get transgenic plants. The main characteristics of Ti plasmid are: Size of the plasmid is ~ 250kbp

Video: pUC19 - Chemie-Schul

Addgene: pUC19 Sequence

Kohlhernie ist eine typische Fruchtfolgekrankheit, die durch den bodenbürtigen Parasiten Plasmodiophora brassicae verursacht wird. Der Parasit verursacht Gewebewucherungen. Die in den Wucherungen gebildeten Dauersporen können im Boden viele Jahre überleben. Ein Kohlhernie Befall kann durch eine weit gestellte Fruchtfolge weitgehend verhindert werden The replicon is comprised of the origin of replication ( ori) and all of its control elements. The ori is the place where DNA replication begins, enabling a plasmid to reproduce itself as it must to survive within cells. The replicons of plasmids are generally different from the those used to replicate the host's chromosomal DNA, but they still.

How to facilitate your research on the 2019 Novel Coronavirus (SARS-CoV-2) Webinar summary: Learn about IDT's high-quality line of genomic reagents that can be used to facilitate your research of COVID-19, caused by the novel coronavirus, 2019-nCoV (officially named SARS-CoV-2). All DECODED articles. Get up to date Figure 18-28 Cloning a Gene in the Plasmid Vector pUC19. (b) Insertion of foreign DNA into the plasmid. (1) The plasmid is cleaved with a restriction enzyme known to recognize a single site, in this case one that is within the lacZ gene. (2) The same enzyme is used to cleave a foreign DNA molecule containing a gene of interest, thereby. The certificate of analysis for that lot of Escherichia coli ER2566 NEB (pUC19-MspA1M, pACYC T7ter-MspA1Ir) ( PTA-4046) is not currently available online. Complete this form to request this certificate of analysis. We have received your request for this certificate of analysis. We will contact you as soon as possible Figure: A Plasmid Map of pUC19: pUC19 is one of a series of plasmid cloning vectors created by Messing and co-workers in the University of California. The p in its name stands for plasmid and UC represents the University in which it was created. It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the most widely used vector molecules as the recombinants, or the cells. Plasmid pUC19 was extracted from E. coli DH5α. pUC19 (2686 bp) is a commercially available E. coli vector carrying an ampicillin resistance gene (amp R, 861 bp). One hundred μL of E. coli DH5α mid-exponential growth phase culture in LB broth medium with 50 mg/L of ampicillin was transferred into 150 mL of LB broth medium containing 50 mg/L of ampicillin and incubated overnight (200 rpm, 37.

pUC19 (Invitrogen, cat. no. 15364-011) or any preferred cloning plasmid. PCR primers or oligos for sgRNA construction are listed in Table 1 and in Supplementary Data 1. Primers longer than 60 bp. The Basic Local Alignment Search Tool (BLAST) finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families pUC19 plasmids carrying LexA-regulated promoters were generated by amplifying the promoter sequences from an E. coli promoter library 72. Insertions were made into the multiple cloning site on. types Plasmids are classified 1. by their ability to be transferred to other bacteria. 1. Conjugative. The . sexual transfer. of plasmids to another bacterium through a pilus. those plasmids possess the 25 genes required fo Aldevron has been perfecting plasmid DNA production for more than 20 years, using proprietary technology to manufacture DNA for a wide range of research, pre..

pUC19 - EcoliWik

Ativeer Tech - A Complete Laboratory Solution. Laboratory Equipment, Glassware, Chemical, Medical Equipment pUC19 was shown to ligate more efficiently than NdeI-digested pUC19. Upon close examination, HindIII-digested pUC19 could also ligate intermolecularly and intramolecularly whereas NdeI-digested pUC19 can only ligate intermolecularly. Ligation of HindIII-digested pUC19 yielded monomeric and multi-meric, circular plasmid wit

pUC18/pUC19 (unverdaut), DNA-Marker Genaxxon bioscience

The isolation of pUC19 and pBR322 from E. coli DH5α strains containing these plasmids was achieved using the Promega Wizard Kit. Concentrations of 75.0 ng/µl of pUC19 and 76.5ng/µl of pBR322 were obtained according to absorbance readings at A260 and A280. The isolated pUC19 and HindIII-digested lambda were then digested with NdeI and gel. Sequence Extractor: Main | Features | Help | Download | License | About: Sequence Extractor generates a clickable restriction map and PCR primer map of a DNA sequence. Protein translations and intron/exon boundaries are also shown QC report and Control plasmid ( pUC19,10-4 μg/μl, stored at -20˚C ~-70˚C ) High 108 HIT: 108 Efficiency ( Ready to Use ) 10 viaIs of 100 μl high 108 HIT competent cells ( should be stored at -70˚C ~-80˚C ) QC report and Control plasmid ( pUC19,10-4 μg/μl, stored at -20˚C ~-70˚C ) Super 109 HIT: 109 Efficiency ( Ready to Use ) 10 viaIs of 100 μl Super 109 HIT competent cells. PSF-CMV-PUC19 - CMV PUC19 MCS PLASMID plasmid vector for molecular cloning; Synonyms: 発現ベクター,プラスミドベクター,ベクター,クローニングベクター,分子クローニングベクター,プラスミド; find Sigma-Aldrich-OGS106 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldric

Будова. Вектор pUC19 містить N-кінцевий фрагмент гена lacZ кишкової палички, що кодує β-галактозидазу, яка здатна розщеплювати глікозидний зв'язок. Сайт (ділянка) множинного клонування, або полілінкер (MCS) локалізується на. The k value for pUC19 transforming activity loss was similarly much lower than the k calculated for cyclobutane-pyrimidine dimer (CPD) formation in the entire plasmid. These results indicate the significant role of CPD repair in the host cells. The degradation rate constants (k) of amp R measured by qPCR increased with increasing target amplicon size (192-851 bp) and were close to the k.

pUC18 and pUC19 vectors - Takara Bi

Fig. 10: EL222-pUC19. The gene coding for NLS-EL222-vp16 is between XbaI and BamHI restriction sites in order to be integrated in the Limonene Synthase-pUC19 or the brazzein-CrGES-pUC19. We used the terminator ADH1 and the promoter pPGK1 for the gene NLS-EL222-vp16. The promoter C120 is between the restriction sites of XmaI and SalI in order to. 2005.11.06 AML Version 1.2 1 Site Directed Mutagenesis Protocol Background ThisprotocolisbasedonacombinationoftheStratageneQuikchangeprotocolandinformationgleane Translations in context of PUC19 vector in English-Chinese from Reverso Context: In order to connect with PUC19 vector, the gene fragments of heat shock protein HSP70 were linked with the double-stranded artificial joints With EcoR I restriction site Control Plasmid (pUC19) 5 μl (10-4 μg/μl) # FYE607-96WL (96 preps) Efficiency >1 x 10 8 cfu/μg: 50 μl/well: 96 wells # FYE608-96WL (96 preps) Efficiency >3 x 10 8 cfu/μg: 50 μl/well: 96 wells # FYE609-96WL (96 preps) Efficiency >1 x 10 9 cfu/μg: 50 μl/well: 96 wells: Support/ Documents. Manual: ECOS Card Headquater/Company. Rm. 2, 5F., No.51, Sec. 2, Keelung Rd., Xinyi Dist., Taipei. Competent cell type: Chemically Competent. Derivative of: DH5-alpha. Species: E. coli. Format: Tubes or Plates (96 x 20 µl) Transformation efficiency: ≥1 x 10 9 cfu/µg pUC19 DNA. Blue/white screening: Yes. Storage/Handling: This product may be shipped on dry ice

What is pUC19 plasmid? - Bioinformatics Indi

Amazon.de/Fashion: Kostenlose Lieferung und Rückgabe. pUC19 Molekularbiologie T-Shirt. Jetzt bestellen A Plasmid Map of pUC19: pUC19 is one of a series of plasmid cloning vectors created by Messing and co-workers in the University of California. The p in its name stands for plasmid and UC represents the University in which it was created. It is a circular double stranded DNA and has 2686 base pairs. pUC19 is one of the most widely used vector molecules as the recombinants, or the cells into. Use NEBcloner to find the right products and protocols for each in your traditional cloning workflow, including double digestion buffers

New England Biolabs (UK) Ltd - pUC19 Vecto

For example, if 10 pg of pUC19 yields 50 colonies when 50 µl of a 1:100 dilution is plated, then: CFU/µg = 50 CFU × 1 × 106 pg × 1 ml × 102 = 1.0 × 1010 10 pg µg 0.05 ml plated Page 2 Transformation Procedure pUC19 control DNA (10 pg/µl) is provided to determine transformation efficiency. Use sample DNA that is free of phenol, ethanol, salts, protein, and detergents to obtain maximum. Names, nicknames and username ideas for puc19. Thousands of randomly generated ideas - funny, weird, creative, fancy, badass and more pUC19-derived : MoClo Toolkit : MK969 : pAK : Addgene : pET325Cm-YGFP : pET-28 a (+) derived : SGI-DNA : Inserts. Our part collection provides an array of modular cloning (MoClo) compatible parts that can be used for predictable expression of genes across different bacterial species. Our collection consists of these inserts or combinations thereof. To ensure the safety of our project we made.

pUC19 Sequence and MappUC19 Vector and its Features - Usefulness in Cloning